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SUMMARY The target of the present work was a study of the oxidation-reduction system of the organism damaged with ionizing radiation. The parameters chosen for examination were: the superoxide dismutase activity in erythrocytes, the concentration of reduced glutathione in erythrocytes, the activity of glutathione peroxidase in the total blood, the concentration of malondialdehyde in the blood plasma, and the antioxidant capacity of blood plasma. These parameters were monitored in four groups (patients after radiotherapy, volunteers whose blood was submitted to in vitro irradiation, employees of a nuclear power plant, and a group of healthy persons). A statistically significant decrease in the GSHPx activity was measured in patients after radiotherapy and in employees of the nuclear power plant. Results of processing the data obtained by the method of multidimensional analysis of variance suggest that the dynamics of the changes is comparable in the group of patients after radiotherapy and employees of the nuclear power plant on the one hand, and in the control group and group of healthy volunteers whose blood was subjected to in vitro irradiation, on the other. The study demonstrated changes concerning certain parameters of the oxidation-reduction system after the action of ionizing radiation. KEYWORDS antioxidant system; blood plasma; ionizing radiation REFERENCES Bhattathiri VN, Sreelekha TT, Sebastian P, Remani P, Chandini R, Vijayakumar T, Nair MK: Influence of plasma GSH level on acute radiation mucositis of the oral cavity. Int. J. Radiat. Oncol. Biol. Phys. 29:383-386, 1994. Bukan N, Guney Y, Hicsonmez A, Bilgihan A: Antioxidant tolerance of kidney after irradiation. Indian. J. Exp. Biol. 41: 267-269, 2003. Burlakova EB, Naidikh VI: The Effects of Low Dose Radiation. VSP, Utrecht, Boston, 2004. Butomo NV, Grebenyuk AN, Legeza VI: Basis of medical radiobiology (in Russian). Foliant, Sankt Peterburg, 2004. Czapski G: Nature of oxygen containing radicals in radiation chemistry and photochemistry of aqueous solutions. Annual Progress Report, September 1979 – July 1980. Technical Report, Hebrew Univ., Jerusalem, Israel, 1980. Deger Y, Dede S, Belge A, Mert N, Kahraman T, Alkan M: Effects of X-ray radiation on lipid peroxidation and antioxidant systems in rabbits treated with antioxidant compounds. Biol. Trace Elem. Res. 94:149-156, 2003. Groen HJ, Meijer C, De Vries EG, Mulder NH: Red blood cell glutathione levels in lung cancer patients treated by radiation and continuously infused carboplatin. Anticancer Res. 16:1033-1037, 1996. Jadhav GK, Bhanumathi P, Uma Devi P, Seetharamaiah T, Vidyasagar MS, Rao KK, Hospet CS, Salomon JG: Possible role of glutathione in predicting radiotherapy response of cervix cancer. Int. J. Radiat. Oncol. Biol. Phys. 41:3-5, 1998. Jin LH, Liu CF, Zeng Y: Protective effects of puerarin on radiation injury of experimental rats. Zhoní Xi Yi Jie He Xue Bao. 3:43-45, 2005. Kalinina EV, Novichkova MD, Charkova EM, Koppel' MA, Komisarova IA: Increase in radiation resistance of an organism after administration of metabolic drug MR-33. Radiats. Biol. Radioecol. 39:272-276, 1999. Kandasamy SB, Kumar KS, Hartus HS: Involvement of superoxide dismutase and glutathione peroxidase in attenuation of radiation-induced hyperthermia by interleukin-1 alpha in rats. Brain Res. 606:106-110, 1993. Kaya H, Delibas N, Serteser M, Ulukaya E, Ozkaya O: The effect of melatonin on lipid peroxidation during radiotherapy in female rats. Strahlenther. Onkol. 175:285-288, 1999. Kojima S, Matsuki O, Nomada T, Shimura N, Kubodera A, Yamaoka K, Tanooka H, Wakasugi H, Honda Y, Honda S, Sasaki T: Localization of glutathione and induction of glutathione synthesis-related proteins in mouse brain by low doses of gamma-rays. Brain Res. 808:262-269, 1998. Kojima S, Teshima K, Yamaoka K: Mechanisms involved in the elevation of glutathione in RAW 264.7 cells exposed to low doses of gamma-rays. Anticancer Res. 20:1589-1594, 2000. Kumar KS, Sancho AM, Weiss JF: A Novel interaction of diethyldithiocarbamate with the glutathione/glutathione peroxidase system. Int. J. Radiat. Oncol. Biol. Phys. 8:1463-1467, 1986. Lefevre G, Beljean-Leymarie M, Beyerle F: Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances. Ann. Biol. Clin. (Paris). 56:395-418, 1998. Marchenko MM, Kopyl'chuk GP, Grigor'eva EV: Influence of small doses of fractionated X-ray radiation on the activity of histone-specific proteases and the content of malonic dialdehyde in chromatin of the cells of rat liver. Radiats. Biol. Radioecol. 42:488-491, 2002. Racek J, Kučera M, Holeček V, Trefil L, Pašek Z: Effects of vitamin and trace element supplements on parameters of antioxidant protection in patients with rheumatic diseases (in Czech). Klin. Biochem. Metab. 7:239-242, 1999. Racek J: Oxidative stress and possibilities of its involvement (in Czech). Galén, Praha, 2004, ISBN 80-7262-2315. Russanov E, Balevska P, Leutshev S: X-irradiation effects on lipid peroxidation and superoxide dismutase and catalase activities in copper deficient and copper loaded rat liver. Acta Physiol. Pharmacol. Bulg. 5:73-78, 1979. Štípek S: Antioxidants and free radicals in health and disease (in Czech). Grada Publishing, Praha, 2000. Steinerová A, Racek J, Holeček V: Effects of administering fenofibrate (Lipanthyl 100) and alpha-tocoferol (vitamin E) on lipid level and superoxide dismutase activity (in Czech). Klin. Biochem. Metab. 7:106-108, 1995. Vartanyan LS, Gurevich S, Kozachenko AI, Navlech LG, Burlakova EB: Age-related peculiarities of effect of low dose ionizing radiation on blood antioxidant enzyme system status in Chernobyl's accident liquidation participant. Adv. Gerontol. 14: 48-54, 2004. Wachowicz B, Krajewski T, Wodzinowska B, Reliszka L: Effects of gamma radiation on pig platelet function. Environ. Res. 34: 1-7, 1984. Wang Z, Zeng L, Xiao Y, Lu S, Gao X: Protective effects of green tea on mice with the irradiating damage induced by gamma-ray. Sichuan Da Xue Xue Bao Yi Xue Ban. 34:303-30, 2003. Yamaoka K, Sato EF, Utsumi K: Induction of two species of superoxide dismutase in some organs of rats by low dose X-irradiation. Physiol. Chem. Phys. Med. NMR. 26:205-214, 1994. Yanagisawa T, Urade M, Takahashi Y, Yamamoto Y, Furuyama J: Levels of superoxide dismutases, glutathione, and poly(ADP-ribose) polymerase in radioresistant human KB carcinoma cell line. Jpn. J. Cancer Res. 88: 1070-1077, 1997. Yarmonenko SP, Vaison AA: Radiobiology of humans and animals (in Russian). Vysshaya shkola, Moscow, 2004 |
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