SUMMARY
Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently,
alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor
effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a
dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its
phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at
a concentration of 1–3 micromol/l. Inhibition of the proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in
p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells
treated with alpha-tomatine at concentrations of 0.1-9 mmol/l. Our results thus contribute to the understanding of the anticancer action of
alpha-tomatine.
KEY WORDS
alpha-tomatine; DNA damage; p53; PUMA; leukemia
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