SUMMARY
We evaluate the possibility of using insects as a haematological biomodel and we test phytoestrogen
isoflavanoid, genistein, in Schistocerca gregaria, Periplaneta americana and Dysdercus cingulatus.
Various reactions of haemocytes were found in these three species, both stimulation and inhibition
of haemocyte concentration. The observed data reflected well the possible effects of soy products
described in published studies on human subjects and it seems that future studies on the sensitivity
of insect haemocytes could lead to an alternative haematological biomodel.
KEY WORDS
alternative biomodel; haemocyte; genistein; pre-clinical prediction
REFERENCES
Albertazzi P: Purified phytoestrogens in
postmenopausal bone health: is there a role for
genistein? Climacteric 5:190-196, 2002.
Belcher SM, Zsarnovszky A: Estrogenic
actions in the brain: estrogen, phytoestrogens,
and rapid intracellular signaling mechanisms.
J Pharmacol Exp Ther 299:408-414, 2001.
Berger J: Leucokinetic study. Morphology of the
bone marrow and blood after experimental
induction of marrow hypoplasia by
cyclophosphamide in laboratory rats. Folia
Haematol 107:862-877, 1980.
Berger J: Haematological preclinical recording of
adverse drug reactions - current status,
problems, and needs. Part I: Standardization of
experiments in relation to haematological
examination. Folia Haematol 114:1-15, 1987.
Berger J: Social and ethical issues in exploratory
research of biomodels (In Czech). Kontakt 2:144-150, 2000.
Bouker KB, Hilakivi-Clarke L: Genistein: does
it prevent or promote breast cancer? Environ
Health Perspect 108:701-708, 2000.
Bobrowska-Hagerstand M, Wrobel A, Rychlik B,
Bartosz G, Soderstrom T, Shirataki Y,
Motohashi N, Molnar J, Michalak K,
Hagerstrand H: Monitoring of MRP-like activity
in human erythrocytes: inhibitory effect of
isoflavpnes. Blood Cells Mol Dis 27:894-900, 2001.
Castle EP, Thrasher JB: The role of soy
phytoestrogens in prostate cancer. Urol Clin
North Am 29:71-81, 2002.
Charalambidis ND, Zervas CG,
Lambropoulou M, Katsoris PG, Marmaras VJ: Lipopolysaccharide-stimulated
exocytosis of nonself recognition protein from
insect hemocytes depend on protein tyrosine
phosphorylation. Eur J Cell Biol 67:32-41, 1995.
Delclos KB, Bucci TJ, Lomax LG, Latendresse JR, Warbritton A, Weis CC,
Newbold RR: Effects of dietary genistein
exposure during development on male and
female CD (Sprague-Dawley) rats. Reprod
Toxicol 15:647-663, 2001.
Doerge DR, Sheehen DM: Goitrogenic and
estrogenic activity of soy isoflavones. Environ
Health Perspect 110, Suppl 3:349-353, 2002.
Diel P, Smolnikar K, Schulz T, Laudenbach-Leschowski U, Michna H, Vollmer G:
Phytoestrogens and carcinogenesis-differential
effects of genistein in experimental models of
normal and malignant rat endometrium. Hum
Reprod 16:997-1006, 2001.
Fitzpatrick LA: Soy isoflavones: hope or hype?
Maturitas 44:S21-S29, 2003.
Ghafar MA, Golliday E, Bingham J,
Mansukhani NM, Anasatasiadis AG,
Katz AE: Regression of prostate cancer
following administration of Genistein Combined
Polyccharide (GCP), a nutritional supplement: a
case report. J Alterm Complement Med 8:493-497, 2002.
Gottstein N, Ewins BA, Eccleston C,
Hubbard GP, Kavanagh IC, Minihane AM,
Weinberg PD, Rimbach G: Effect of genistein
and daidzein on platelet aggregation and
monocyte and endothelial function. Br J Nutr
89:607-616, 2003.
Hwang J, Wang J, Morazzoni P, Hodis HN,
Sevanian A: The phytoestrogen equol increases
nitric oxide availability by inhibiting superoxide
production: an antioxidant mechanism for cellmediated
LDL modification. Free Radic Biol
Med 34:1271-1282, 2003.
Horvathova K, Vachalkova A, Novotny L:
Flavonoids as chemoprotective agents in
civilization diseases. Neoplasma 48:435-441, 2001.
Kinoshita T, Yokota T, Arai K, Miyajima A:
Suppression of apoptotic death in hematopoietic
cells by signaling through the IL3/GM-CSF
receptors. EMBO J 14:266-275, 1995.
Lee VM, Quinn PA, Jennings SC, Ng LL:
NADPH oxidase activity in preeclampsia with
immortalized lymphoblasts used as models.
Hypertension 41:925-931, 2003.
Misra RR, Hursting SD, Perkins SN,
Sathyamoorthy N, Mirsalis JC, Riccio ES,
Crowell JA: Genotoxicity and carcinogenicity
studies of soy isoflavones. Int J Toxicol 21:277-285, 2002.
Mortelmans K, Zeiger E: The Ames
Salmonella/microsome mutagenicity assay.
Mutat Res 455:29-60, 2000.
Okazaki K, Okazaki S, Nakamura H, Kitamura Y,
Hatayama K, Wakabayashi S, Tsuda T,
Katsumata T, Hishikawa A, Hirose M:
A repeated 28-day oral dose toxicity study of
genistein in rats, based on the 'Enhanced OECD
Test Guidline 407' for screening endocrine-discrupting
chemicals. Arch Toxicol 76:553-559, 2002.
Park KY, Jung KO, Rhee SH, Choi YH:
Antimutagenic effects of doenjang (Korean
fermented soypaste) and its active compounds.
Mutat Res 523-524:43-53, 2003.
Po LS, Wang TT, Chen ZY, Leung LK:
Genistein-induced apoptosis in MCF-7 cells
involves changes in Bak and Bcl-x without
evidence of anti-oestrogenic effects. Br J Nutr
88:463-469, 2002.
Rickard DJ, Monroe DG, Ruesink TJ, Khosla S,
Riggs BL, Spelsberg TC: Phytoestrogen
genistein acts as an estrogen agonist on human
osteoblastic cells through estrogen receptors
alpha and beta. J Cell Biochem 89:633-646, 2003.
Scholz S, Gutzeit HO: Lasting effects of xenoand
phytoestrogens on sex differentiation and
reproduction of fish. Environ Sci 8:57-73, 2001.
Seike N, Wanibuchi H, Morimura K, Weil M,
Nishikawa T, Hirata K, Yoshikawa J,
Fukushima S: Enhancement of lung
carcinogenesis by nonylphenol and genistein in a
F344 rat multiorgan carcinogenesis model.
Canver Lett 192:25-36, 2003.
Song M, Gutzeit HO: Effect of 17-alpha-ethynyloestradiol on germ germ cell proliferation
in organ and primary culture of medaka (Oryzias
latipes) testis. Develop Growth Differ, in press,
2003a.
Song M, Gutzeit HO: Primary culture of
medaka (Oryzias latipes) testis: a test system for
the analysis of cell proliferation and
differentiation. Cell Tissue Res (in press)
2003b.
Steele VE, Pereira MA, Sigman CC,
Kelloff GJ: Cancer chemoprevention agent
development strategies for genistein. J Nutr
125, Suppl. 3:713S-716S, 1995.
Yellayi S, Naaz A, Szewczykowski MA, Sato T,
Woods JA, Chang J, Segre M, Allred CD,
Helferich WG, Cooke PS: The phytoestrogen
genistein induces thymic and immune changes:
A human health concern? Proc Nat Acad Sci
USA 99:7616-7621, 2002.
Yellayi S, Naaz A, Szewczykowski MA, Sato T,
Woods JA, Chang J, Segre M, Allred CD, Helfrich WG, Cooke PS: The phytoestrogen
genistein suppresses cell-mediate immunity in
mice. J Endocrinol 176:267-274, 2003.
Zavodnik LB, Zavodnik IB, Lapshina EA,
Shkodlich AP, Gyszewska M, Buko VU:
Hypochlorus acid-induced lysis of human
erythrocytes. Inhibition of cellular damage by the
isoflavonoid genistein-8-C-glucoside.
Biochemistry 65:946-951, 2000.
|
CITED
Picmonova V, Berger J. Genistein effects on haematoimmune cells in a newly developed alternative toxicological model. Exp Toxicol Pathol. 64: 411-415, 2012.
Berger J. Alternative haematotoxicological testing. J Appl Biomed. 8: 19-22, 2010.
Berger J. Preclinical testing on insects predicts human haematotoxic potentials. Lab Animals. 43: 328-332, 2009.
Berger J, Slavickova K. Morphological characterization of hemocytes in the adult linden bug, Pyrrhocoris apterus (L.) (Heteroptera). Zoological Stud. 47: 466-472, 2008.
Gelbic I, Strbackova J, Berger J. Influence of metyrapone on the morphology of hemocytes of the Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Zoological Stud. 45: 371-377, 2006.
Berger J. Current ethical problems in cell biology. Conference Information: 6th Cells Conference 2005, Oct 24-26, 2005 Univ S Bohemia, Ceske Budejovice, Czech Republic. Adv Cell Biol Mol Biol. p. 1-11, 2005.
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