SUMMARY
The epidermal stem cell plays a pivotal role in the function of squamous epithelia in physiological
as well as in pathological conditions such as cancer. This review summarizes data about the
glycobiology of normal squamous epithelia and related tumors with respect to epithelial cell
differentiation and search for a glycophenotype specific for epidermal stem cells using labeled
plant and endogenous lectins. Although the glycophenotype typical for epithelial cells at the stage
of low differentiation level were found, no typical cell surface saccharidic markers of stem cells
were detected. The nuclear binding of galectin-1 seems to be specific for the keratinocyte
population prepared from hair follicles enriched for multipotent stem cells. The close topographical
relationship of nuclear galectin-1 binding sites with SC35 splicing factor suggests some role for
these glycoepitopes in pre-mRNA splicing. The data shown in this paper can be employed for
diagnostic purposes and for cell therapy of skin defects and indicate the importance of the use of
endogenous lectins as probes in biology and medicine.
KEY WORDS
epidermal stem cell; glycocode; endogenous lectin-galectin-carcinoma
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