SUMMARY
According to the free radical theory of aging, oxidative stress plays a key role in the senescent decline of
the immune system, and therefore antioxidants may provide an effective therapy in order to protect the
immune function against oxidative damage. Accordingly, the purpose of this study was to investigate the
favourable effect of a diet supplemented with a glutathione precursor - namely the antioxidant
N-acetylcysteine (NAC, 0.1% w/w) - on several functions of the leucocytes from prematurely-aging
mice (PAM). In adult PAM, the immune functions studied showed values similar to those of older mice,
and worse than those found in adult non prematurely-aging mice (NPAM). A short-term ingestion
(4 weeks) of NAC, although unable to influence the NK activity, the IL-2 release or the glutathione
content of the peritoneal leucocytes of PAM, resulted in an improvement of other very important
leucocyte functions such as the lymphoproliferative response to the mitogen Con A, and IL-1beta and
TNFalpha release.
KEY WORDS
antioxidant; glutathione; leucocytes; lymphoproliferation; N-acetylcysteine
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CITED
De la Fuente M, Hernanz A. The antioxidant N-acetylcysteine in vitro improves several functions of peritoneal leucocytes from old mice approaching their values to those of adult animals. J Appl Biomed. 10: 79-90, 2012.
De la Fuente M, Hernanz A, Viniegra S, Miguel J. Sulfur-containing antioxidants increase in vitro several functions of lymphocytes from mice. Int Immunopharm. 11: 661-669, 2011.
De la Fuente M, Miquel J. An Update of the Oxidation-Inflammation Theory of Aging: The Involvement of the Immune System in Oxi-Inflamm-Aging. Curr Pharmaceut Design. 15: 3003-3026, 2009.
Arranz L, Fernandez C, Rodriguez A, Ribera JM, De La Fuente M. The glutathione precursor N-acetylcysteine improves immune function in postmenopausal women. Free Radical Biol Med. 45: 1252-1262, 2008.
Arranz L, Fernandez C, Rodriguez, A, Ribera JM, De La Fuente M. Changes with ageing in immune cell and plasma glutathione levels. Effect of N-acetylcysteine administration. Rev Espanola de Geriatria y Gerontologia. 42: 96-102, 2007.
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