SUMMARY
The present study was undertaken to provide more information on the nucleolar size, number and
distribution of AgNORs (silver stained nucleolus organizer regions) in nucleolar bodies of aging
leukemic granulocytic progenitor cells represented by cultured K562 cells. In these aging and starving
cells without further differentiation and with translocated AgNORs to the nucleolar periphery, the
diameter of the nucleolar size was reduced together with a reduction in the number of these nucleolar
compartments. However, after re-feeding cell cultures, the nucleolar size increased again. Similarly, the
number of AgNORs also increased and was distributed in the whole nucleolar body as in control cells.
Such cells apparently entered into the cycle and proliferation documented by the re-occurrence of mitotic
divisions in studied cultures, an increased number of AgNORs and increased nuclear size. This
observation suggests that early granulocytic precursors with translocated nucleolar AgNORs might
represent a transitionally quiescent population of cells which may return to a fully proliferating state.
KEY WORDS
AgNORs; intranucleolar translocation; nucleolar size; K 562 cells
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Smetana K. The nucleolus through the years. J Appl Biomed. 9: 119-127, 2011.
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