SUMMARY
In the present study we have investigated the anticarcinogenic property of diallyl disulphide (DADS) on
hepatic thiobarbituric acid reactive substances (TBARS) and antioxidants such as reduced glutathione
(GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase in control,
N-nitrosodiethylamine and diallyl disulphide treated rats. The levels of TBARS and activities of SOD and
catalase were decreased in NDEA treated rats whereas GSH and GPx tended to increase in carcinogenic
rats. Oral administration of DADS (60mg/kg body wt) tends to normalize these variables in liver. This
clearly indicates that DADS could possess chemopreventive effects by modulating the oxidantantioxidant
status of the living system. However, the exact mechanism remains to be elucidated.
KEY WORDS
DADS; hepatocarcinogenesis; lipid peroxidation; antioxidants
REFERENCES
Anis KV, Rajesh-kumar NV, Kuttan R:
Inhibition of chemical carcinogenesis by
berberine in rats and mice. J Pharm
Pharmacol 53:763-768, 2001.
Antosiewicz HA, Singh SV: Signal transduction
pathways leading to cell cycle arrest and
apoptosis induction in cancer cells cycle arrest
and apoptosis induction in cancer cells by
Allium vegetable derived organosulfur
compounds: a review. Mutation Res 55:121-131, 2004.
Banakar MC, Paramasivan SK, Chattopadhyay
MB et al.: 1alpha, 25-dihydroxyvitamin D3
prevents DNA damage and restores antioxidant
enzymes in rat hepatocarcinogenesis induced
by diethylnitrosamine and promoted by
Phenobarbital. World J Gastroenterol
10:1268-1275, 2004.
Block E: The chemistry and health benefits of
organosulfur compounds in garlic (Allium
sativum): recent findings. Hyper Nutritious
Foods, Auburn vale, Finland 261-292, 1996.
Cheeseman KH, Margaret C, Proudfoot K et al.:
Studies on lipid peroxidation in normal and
tumor tissues: The Novikoff rat liver tumor.
Biochem J 235:507-514, 1986.
Dianzani MU, Canuto RA, Rossi MA et al.:
Further experiments on lipid peroxidation in
transplanted and experimental hepatomas.
Toxicol Pathol 12:189-199, 1984.
Ellman GC: Tissue sulfhydryl groups. Arch
Biochem Biopsy 82:70-77, 1959.
Germain E, Augur J, Ginies C et al.: In vivo
mechanism of diallyl disulphide in the rat: identification of two new metabolites.
Xenobiotica 32:1127-1138.
Guyonnet D, Belloir C, Suschetet M et al.:
Antimutagenic activity of organosulfor
compounds from Allium is associated with
phase II enzyme inductions. Mutation Res
495:135-145, 2001.
Hayes JD, Pulford DJ: The GST supergene
family: regulation of GST and the contribution
of isoenzymes to cancer chemoprevention and
resistance. Crit Rev Biochem Mol Biol 30:445-600, 1995.
Kakkar P, Das B, Viswanathan PN: A modified
spectroscopic assay of superoxide dismutase.
Indian J Biochem Biophys 21:130-132, 1984.
Lawson LD: The composition and chemistry of
garlic cloves and processed garlic. Garlic. The
Science and Therapeutic application of Allium
sativum L. and related species. Williams and
Wilkins, Baltimore, MD 37-107, 1996.
Lu HF, Sue CC, Yu CS et al.: Diallyl disulfide
(DADS) induced apoptosis undergo caspases
activity in human bladder cancer T24 cells.
Food and Chem Toxicol 42:1543-1552, 2004.
Mirunalini S, Subramanian P: Temporal
oscillations of thyroid hormones in long term
melatonin treated rats. Pharmazie 60:52-56, 2005.
Mignard HD, Suschetet M, Berges R et al.:
Inhibition of aflatoxin B1 and NNitrosodiethylamine - induced liver
preneoplastic foci in rats fed naturally
occurring allyl sulfides. Nutrition 25:1-70, 1996.
Nakagami K, Uchida T, Ohwada S: Increased
choline kinase activity in 1,2-dimethyl
hydrazine induced rat colon cancer. Jpn J
Cancer Res 90:1212-1217, 1999.
Puscas I, Baican M, Coltan M et al.: Erythrocyte
superoxide dismutase activity in patients with
digestive cancer: Adjuvant diagnosis test.
Cancer Lett 143:95-98, 1999.
Rajesh-kumar NV, Kuttan R: Inhibition of N-Nitrosodiethylamine
induced hepatocarcinogenesis by picroliv. J Exp Clin
Cancer Res 19:459-465, 2000.
Rossen RT et al.: Determination of allicin, S-allyl
cysteine and volatile metabolites of garlic in
breath, plasma or stimulated gastric fluids. J
Nutr 131:968-971, 2001.
Rotruck JT, Pope AL, Ganther HE: Selenium: biochemical role as a component of glutathione
peroxidase purification assay. Science
179:588-590, 1973.
Schaff Z, Kovalszky P, Nagy P et al.: Human and
experimental carcinogenesis. Scand J
Gastroenterol 228:90-97, 1998.
Singh R, Singh RK, Mahdi AA et al.: Circadian
periodicity of plasma lipid peroxides and other
putative markers in gynecological
malignancies. In vivo 17:593-600, 2003.
Sinha AK: Colorimetric assay of catalase. Anal
Biochem 47:389-394, 1972.
Sun Y: Free radicals, antioxidant enzymes and
carcinogenesis. Free Rad Med Biol 8:583-599, 1990.
Sundaram SG, Milner JA: Diallyl Disulfide
induces apoptosis of human colon tumor cells.
Carcinogenesis 17:669-673, 1996.
Sunderasen S, Subramanian P: Evaluation of
chemopreventive potential of garlic extract on
N-nitrosodiethylamine induced
hepatocarcinoma in rats. Pharmaceutical Biol
40:548-551, 2002.
Sunderasen S, Subramanian P: S-allyl cysteine
inhibits circulatory lipid peroxidation and
promotes antioxidants in N-nitrosodiethylamine
induced carcinogenesis. Pol J Pharmacol 55:37-42, 2003a.
Sunderasen S, Subramanian P: Garlic modulates
lipid peroxidation and antioxidant status during
N-nitrosodiethylamine-induced hepatic
tumorigenesis. Plant Foods for Human
Nutrition 58:1-8, 2003b.
Verna L, Whysner J, Williams GM: N-Nitrosodiethylamine
Mechanistic data and risk
assessment: bioactivation, DNA-adduct
formation, mutagenicity and tumor initiation.
Pharmacol Ther 71:57-81, 1996.
Wattenberg LW, Sparnins VL, Barany G:
Inhibition of N-Nitrosodiethylamine
carcinogenesis in mice by naturally occurring
organosulfur compounds and monoterepenes.
Cancer Res 49:2689-2692, 1982.
Wu CC, Sheen LY, Chen HW et al.: Effects of
organosulfur compounds from garlic oil on the
antioxidant system in rat liver and red blood
cells. Food and Chem Toxicol 39:563-569, 2001.
Wu X, Kassie F, Mersch-Sundermann V:
Induction of apoptosis in tumor cells by
naturally occurring sulfur containing
compounds. Mutation Res 589:81-102, 2004.
Yagi K: Lipid peroxides and human diseases.
Chem Phys Lipids 45:337-351, 1987.
|
CITED
Subramanian P, Kumaravel P, Manivasagam T. Role of biological clocks in cancer processes and chronotherapy. Biol Rhytm Res. 41: 391-402, 2010.
Zoghbi MDB, Oliveira J, Guilhon GMSP. The genus Mansoa (Bignoniaceae): a source of organosulfur compounds. Rev Bras Farmac-Braz J Pharmac. 19: 795-804, 2009.
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