SUMMARY
Organophosphorus pesticides such as parathion or chlorpyrifos are substances used worldwide for
agricultural purposes. These compounds are able to inhibit an enzyme acetylcholinesterase (AChE; EC
3.1.1.7) by phosphorylation in its active site. AChE reactivators and anticholinergics are generally used as
antidotes in the case of intoxication by these agents. In this work, the reactivation potency of nine
structurally different AChE reactivators was tested in vitro. Chlorpyrifos was chosen as an appropriate
member of the pesticide family. The result is that bisquaternary reactivators with two oxime groups in
position four at the pyridinium rings (trimedoxime and K074) seem to be the most potent reactivators of
chlorpyrifos-inhibited AChE.
KEY WORDS
acetylcholinesterase; reactivator; pesticide; organophosphate
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