SUMMARY
The effect of nucleotides in the newborn is a determinant in this first stage of life, and their correct
level in breastmilk is vital. We have designed a new method for the assay of nucleotides in milk by
capillary electrophoresis (CE) after acid hydrolysis. Breastmilk samples were collected from healthy
mothers (ages, 25-35 years) of one month lactation, and stored at -20 °C. The duplicated samples
were dissociated by acidic hydrolysis (HClO4) and the CE assay was performed in an uncoated
fused-silica capillary using an alkaline (borate) electrophoretic separation system.
The method gave good recoveries of 5´-mononucleotides. Under the conditions used, the actual
CE analysis time was less than 20 minutes. The physiologically and nutritionally important
nucleotides were detected at concentrations of 387 microg/100ml for UMP-5P, 385.3 microg/100ml for
AMP-5P, 67 microg/100ml for CMP-5P, 172 microg/100ml for TMP-5P and 315 microg/100ml for GMP-5P.
Nucleotides are a significant nutrient in infant growth, and capillary electrophoresis is a sensitive
and efficient tool for the assay of nucleotides with a purine or pyrimidine base in breastmilk.
KEY WORDS
nucleotides; capillary electrophoresis; breastmilk
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Ding XJ Li Y Song BH, Zhao S, Wang Z. Determination of 5 '-mononucleotides in infant formula by capillary electrophoresis with ultraviolet detection. Dairy Sci
Technol. 91: 759-770, 2011.
Chen XJ, Yang FQ, Wang YT, Li SP. CE and CEC of nucleosides and nucleotides in food materials. Electrophoresis. 31: 2092-2105, 2010.
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