SUMMARY
The purpose of our study is to evaluate the protective effect of diphenyl dimethyl bicarboxylate (DDB) in
combination with some antioxidants, namely vitamin C (V.C), vitamin E (V.E), and selenium (Se), in
liver damage induced by carbon tetrachloride (0.2 ml/kg body weight). This was done by monitoring the
liver total and fractional lactate dehydrogenase (LDH) activities. The results revealed a significant
increase in the activity of liver total LDH activity in CCl4 - intoxicated rats with a significant increase in
both LDH3 and 4 and a significant decrease in LDH5. LDH2 disappeared after CCl4 treatment and
neither DDB nor its combinations could restore this permanent change. DDB alone significantly
decreased the CCl4-raised total LDH and LDH4, but still far from the control and failed to correct LDH3
and 5 variations. A combination of DDB and V.C, V.E and Se showed the best corrective potential in
both total LDH and LDH3 activities, without correcting the increased LDH4, nor the decreased LDH5
isoenzyme. Although this combination was previously reported to correct liver function disturbances, it
seems that CCl4 and consequently hepatitis C may induce some irreversible, non-curable changes by
DDB or even by additional antioxidants. Its clinical usefulness seems to be through different metabolic
pathways, not including correction of LDH disturbances, which necessitates additional investigation for
other adjunct medicines for treating liver fibrosis in clinical practice.
KEY WORDS
LDH isoenzymes; liver fibrosis; DDB; CCI4; rats
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