SUMMARY
Free radicals are believed to play an important role in many pathological states. Consequently
antioxidants are receiving increased attention in medicinal research. As part of studies of the biological
effects of the antidotes against chemical warfare agents currently used in the Czech Armed Forces, eleven
compounds were assayed for their free radical scavenging activity. An optimized PC-controlled sequential
injection analysis (SIA) system with spectrophotometric detection was used for evaluation of the
antioxidation activity of the antidotes. The automated method is based on the known reaction of 2,2´-
diphenyl-1-picrylhydrazyl radical (DPPH) with antioxidants. Only the radical scavenging activity of the
tested compound 2,3-dimercaptopropanol (BAL) was found to be weaker compared to the reference
antioxidant acidum ascorbicum. Other tested antidotes did not possess any DPPH radical scavenging
activity. The results obtained show that a correlation between the required biological activity (prophylaxis
or treatment of intoxications by warfare agents) and possible antioxidation activity of the tested antidotes
is doubtful.
KEY WORDS
antioxidant; radical; analysis; antidote; warfare agent
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