SUMMARY
Cyclooxygenase is the enzyme responsible for the production of prostaglandins (PGs). This cyclooxygenase exists
in two isoforms: cyclooxygenase-1 (COX-1) and cyclooxygense-2 (COX-2). In humans and primates high levels
of COX-2 are detected in the seminal vesicle. Further, the main source of PGs in the semen of these species is
from the seminal vesicle. In rodents, the source of PGs in semen is from the vas deferens and abundant levels
of COX-2 are detected. A direct relation is thought to exist between COX-2 levels and the source of PGs in semen.
Moreover, the role of COX-1 and COX-2 in the seminal vesicle of rodents is obscure. The present study aims at
localizing COX-1 and COX-2 in the seminal vesicle of mice. Immunohistochemical staining and COX activity assay
revealed COX-1 as a dominant isoform in the mouse seminal vesicle. On treatment with nimesulide - a preferential
COX-2 inhibitor - no change in staining intensity and COX activity was observed. The total PG levels also appeared
to be unaltered following nimesulide treatment. This confirms that nimesulide had no effect on COX-1.
The results presented here suggest COX-1 is the dominant isoform in the mouse seminal vesicle and is responsible
for PG synthesis.
KEY WORDS
cyclooxygenase-1; cyclooxygenase-2; mice; nimesulide; prostaglandins; seminal vesicle
REFERENCES
Allport V, Bennett O: Cyclooxygenase enzymes and human labor in therapeutic roles of selective COX-2 inhibitors. In: Vane JR, Botting RM, eds. London: William Harvey Press Pp.252-273, 2001.
Balaji T, Ramanathan M, Menon VP: Localization of cyclooxygenase-2 in mice vas deferens and its effects on fertility upon suppression using nimesulide - A preferential cyclooxygenase-2 inhibitor. Toxicol 234:135-144, 2007a.
Balaji T, Ramanathan M, Menon VP: Localization of cyclooxygenase-2 in mice testis and assessment of its possible through suppressing its expression using nimesulide - a preferential cyclooxygenase-2 inhibitor.Prostaglandins leukot. Essent Fatty acids 76:341-348, 2007b.
Balasubramaniam J: Selective COX-2 inhibitors and nephrotoxicity. Am J Kidney Dis 36:675-676, 2000.
Bernareggi A: Clinical pharmacokinetics of nimesulide. Clin Pharmacokinet 35:247-274, 1998.
Botting RM: Cyclooxygenase past present and future a tribute to John R Vane (1927-2004). J Therm Biol 34:208-219, 2006.
Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 33:497-509, 1957.
Giuliana F, Warner TD: Ex vivo assay to determine the cyclo-oxygenase selectivity of non-steroidal drugs. Brit J Pharmacol 126: 1824-1830, 1999.
Hamilton GS, Kennedy TG: Uterine vascular changes after intrauterine infusion of indomethacin and prostaglandin E2 to rats sensitized for the decidual cell reaction. Biol Reprod 50:757-764, 1995.
Kates M: Isolation, analysis and identification of lipids. In: Work T, Work E, eds. Techniques in lipidology. Amsterdam: Elsevier Pp. 279, 1972.
Kelly RW: Effect of seminal prostaglandins on the metabolism of human spermatozoa. J Reprod Fertil 50:217-222, 1977.
Kelly RW: Prostaglandin synthesis in the male and female reproductive tract. J Reprod Fertil 62:293-304, 1981.
Kelly RW: Immunosuppressive mechanisms in semen: implication of contraception. Hum Reprod 10:1686-1693, 1995.
Kennedy JH, Korn N, Thurston RJ: Prostaglandin levels in seminal plasma and sperm extracts of the domestic turkey, and the effects of cyclooxygenase inhibitors on sperm mobility. Reprod Biol Endocrinol 1:74-87, 2003.
Kirschenbaum A, Liotta DR, Yao S, Xin-Hua L, Klausner AP, Unger P, Shapiro E, Leau I, Levine AC: Immunohistochemical localization of cyclooxygenase-1 and cyclooxygenase-2 in the human fetal and adult male reproductive tracts. J Clin Endocrinol Metab 85:3436?3441, 2000.
Klein LA, Stoff JS: Prostaglandin synthesis is independent of androgen levels in rat male genitalia. J Lab Clin Med 109:402-408, 1987.
Lazarus M, Eguchi N, Matsumoto S, Nagata N, Yana T, Killian GJ, Urade Y: Species specific expression of microsomal Prostaglandin E synthase-1 and cyclooxygenases in male monkey reproductive organs. Prostaglandins Leukot Essent Fatty Acids 71:233-240, 2004.
Leung GPH, Cheung KH, Leung CT, Tsang MW, Wong PYD: Regulation of epididymal principal cell function by basal cell: role of transient receptor potential (Trp) proteins and cyclooxygeanse-1 (COX-1). Mol Cell Endocrinol 216:5-13, 2004.
McKanna JA, Zhang MZ, Wang JL, Cheng HF, Harris RC: Constitutive expression of cyclooxygenase-2 in rat vas deferens. Am J Physiol Regul Integr Comp Physiol 275:227-233, 1988.
Mingatto FE, Rodrigues T, Pigoso AA, Uyemura SA, Curtis C, Santos AC: The critical role of mitochondrial energetic impairment in the toxicity of nimesulide to hepatocytes. J Pharm Exp Ther 303:601-607, 2002.
Monjazeb AM, High KP, Koumenis C, Chilton FH: Inhibitors of arachidonic acid metabolism act synergistically to signal apoptosis in neoplastic cell. Prostaglandins Leukot Essent Fatty Acids 73:463-474, 2005.
Montensinos S, Hallal H, Raussell V, Canesa F, Lopez A: Nimesulide induced acute hepatitis. Gastroenterol Hepatol 24:219-220, 2001.
Morham SG, Langenbach R, Loftin CD, Tiano HF, Voulomanos N, Jennette JC, Mahler JF, Kluckman KD, Ledford A, Lee CA: Prostaglandin synthase-2 gene disruption cause severe renal pathology in the mouse. Cell 83:473-482, 1995.
Neeraja S, Sreenath AS, Reddy PRK, Reddanna P: Expression of cyclooxygenase-2 in rat testis. Reprod Biomed Online 6:300-307, 2003.
Sakurai T, Tamura K, Okamata S, Hara T, Koga H: Possible role of cyclooxygenase II in the acquisition of ovarian luteal function in rodents. Biol Reprod 69:835-842, 2003.
Shafiq N, Malhotra S, Pandhi P: Comparison of non selective cyclooxygenase (cox) inhibitors and selective cox-2 inhibitors on preimplantation loss, post implantation loss and duration of gestation: an experimental study. Contraception 69:71-75, 2004.
Shimizu Y, Yorimitsu A, Maruyama T, Kuboto T, Aso T, Bronson RA: Prostaglandin induce calcium influx in human spermatozoa. Mol Hum Reprod 4:555-561, 1998.
Simmons DL, Botting RM, Hla T: Cyclooxygenase isozymes: the biology of prostaglandin synthesis and inhibition. Pharm Rev 56:387-437, 2004.
Tay VKS, Wang AS, Leow KY, Ong MK, Wong KP, Boelsterli UA: Mitochondrial permeability transition as a source of superoxide anion reduced by the nitroaromatic durg nimesulide in vitro. Free Radical Bio Med 39:945-959, 2005.
Von Euler US: On specific vasodilating and plain muscle stimulating substance from accessory genital glands in man and certain animals (prostaglandin and vestiglandin). J Physiol 88:213-234, 1936.
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CITED
Balaji T, Kumar SS, Ramanathan M, Subramaniam A, Subramanian M. Effect of nimesulide on cytosolic phospholipase A2 activity in male reproductive organs of mice. Biomed (India). 32: 363-368, 2012.
Ugochukwu AP, Ebere OO, Okwuoma A. Effects of nimesulide on testicular functions in prepubertal albino rats. J Basic Clin Physiol Pharmacol. 22: 137-140, 2011.
Welsh M, Moffat L, Jack L, McNeilly A, Saunders PTK, Sharpe RM, Smith LB. Deletion of androgen receptor in the smooth muscle of the seminal vesicles impairs secretory function and alters its responsiveness to exogenous testosterone and estradiol. Endocrinol. 151: 3374-3385, 2010.
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