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<b><font color="#3366FF"><font size=+4>Journal of APPLIED BIOMEDICINE</font></font></b><br>
ISSN 1214-0287 (on-line)<br>
ISSN 1214-021X (printed)
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Volume 6 (2008), No 4, p 203-210
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The effect of L-arginine on serum lipids and nitrite levels, and the number of apoptotic cells, iNOS and eNOS expressions of aorta after the formation of fatty streaks in rabbit
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Mehdi Nematbakhsh, Shaghayegh Haghjooyjavanmard, Farzaneh Mahmoodi, Ali Reza Monajemi
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Address: Mehdi Nematbakhsh , Applied Physiology Research Center and Department of Physiology,
Isfahan University of Medical Sciences, Hezar jerib Avenue, Isfahan, Iran
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<a href="mailto:nematbakhsh@gmail.com">nematbakhsh@gmail.com </a>
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Received 6th May 2008.<br>
Revised 6th November 2008.<br>
Published online 1st December 2008.<br>
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<a name="summary"><b>SUMMARY</b></a><br>
L-arginine is the substrate of nitric oxide production which is involved in the regulation of apoptosis and inflammatory processes. The effect of L-arginine after the formation of fatty streaks (FS) is not fully understood in the hypercholesterolemic model and therefore the main objective of this study was to investigate the effect of L-arginine after fatty streaks were developed in the rabbit aorta.
Eighteen male rabbits were fed a 1% cholesterol diet for four weeks. One third of the animals were sacrificed randomly to verify fatty streak formation in the aorta (phase I). Then the high cholesterol diet was replaced with a normal diet, and the remaining animals (n=12) were divided into two groups (phase II); group 1 (n=6): normal diet and group 2 (n=6): normal diet plus L-arginine (3% in drinking water). The experiment was continued for a further four weeks.
The serum levels of lipids and lipoproteins were increased significantly in phase I (p<0.05), and the fatty streaks score (1�0.44) indicates the formation of lesions in the aorta. In Phase II, no significant differences were observed between the groups for lipids and nitrite concentrations, inducible and endothelial nitric oxide synthase expressions, the number of apoptotic cells, or fatty streak development.
These findings show that the administration of 3% L-arginine for four weeks has no beneficial effect in the developed fatty streaks model.
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<a name="keywords"><b>KEYWORDS</b></a><br>
L-arginine; Nitric oxide; iNOS; eNOs; apoptosis; hypercholesterolemia<br>
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<a name="references"><b>REFERENCES</b></a><br>
Adams MR: Oral L-arginine improves endothelium dependent dilatation and reduces monocyte adhesion to endothelial cells in young men with coronary disease. Atherosclerosis 129: 261-169, 1997.
<br><br>
Bj�rkerud S, Bj�rkerud B: Apoptosis is abundant in human atherosclerotic lesions especially in inflammatory cells (macrophages and T cells; and may contribute to the accumulation of gruel and plaque instability. Am. J. Pathol. 149: 367�380, 1996.
<br><br>
Boger RH: Supplementation of hypercholesterolemic rabbits with L arginine reduces vascular release of superoxide anions and restores NO production Atherosclerosis. 117: 273-284, 1995.
<br><br>
B�ger RH: Dietary L-arginine reduces the progression of atherosclerosis in cholesterol-fed rabbits: comparison with lovastatin. Circulation 96: 1282-1290, 1997.
<br><br>
Brune B: Nitric oxide: NO apoptosis or turning it ON? Cell Death Differ 10: 864-9, 2003.
<br><br>
Clarkson P: Oral L-arginine improves endothelium-dependent dilation in hypercholesterolemic young adults. J. Clin. Invest. 97: 1989-1994, 1996.
<br><br>
Cai H, Harrison DG: Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circ Res. 87: 840-844, 2000.
<br><br>
Cooke JP: Antiatherogenic effects of L-arginine in the hypercholesterolemic rabbit. J Clin Invest 90: 1168-1172, 1992.
Fleming I, Busse R, Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase. Am. J. Physiol. Regul Integr Comp Physiol 284: R1-12, 2003.
<br><br>
Hayashi T, Matsui-Hirai H, Fukatsu A, Sumi D, Kano-Hayashi H, Rani P, Iguchi A: Selective iNOS inhibitor ONO1714 successfully retards the development of high-cholesterol diet induced atherosclerosis by novel mechanism. Atherosclerosis 187: 316-24, 2006.
<br><br>
Hayashi T, Sumi D, Juliet PA, Matsui-Hirai H, Asai-Tanaka Y, Kano H, Fukatsu A, Tsunekawa T, Miyazaki A, Iguchi A, Ignarro LJ: Gene transfer of endothelial NO synthase but not eNOS plus inducible NOS regressed atherosclerosis in rabbits. Cardiovasc. Res. 61: 339-351, 2004.
<br><br>
Jablecka A, Checi?ski P, Krauss H, Micker M, Ast J: The influence of two different doses of L-arginine oral supplementation on nitric oxide (NO) concentration and total antioxidant status (TAS) in atherosclerotic patients. Med. Sci. Monit. 10: CR29-32, 2004.
<br><br>
Kukreja RC, Xi L: eNOS phosphorylation: a pivotal molecular switch in vasodilation and cardioprotection? J. Mol. Cell Cardiol. 42: 280-2 , 2007.
<br><br>
Li CQ, Wogan GN: Nitric oxide as a modulator of apoptosis. Cancer Lett. 226: 1-15, 2005.
<br><br>
Lowenstein CJ, Padalko E: iNOS (NOS2) at a glance J. Cell Sci. 117: 2865-7, 2004.
<br><br>
Braam B, Verhaar MC: Understanding eNOS for pharmacological modulation of endothelial function: a translational view. Curr. Pharm. 13: 1727-40, 2007.
<br><br>
Mensah GA, Ryan US, Hooper WC, Engelgau MM, Callow AD, Kapuku GK, Mantovani A: Vascular endothelium summary statement II: Cardiovascular disease prevention and control. Vascul. Pharmacol. 46: 318-320, 2007.
<br><br>
Minor RL, Myers PR, Guerra R, Bates JN, Harrison DG: Diet-induced atherosclerosis increases the release of nitrogen oxides from rabbit aorta. J. Clin. Invest. 86: 2109-2116, 1990.
<br><br>
Moroe H, Honda H: Comparison of endothelial function in the carotid artery between normal and short-term hypercholesterolemic rabbits. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 144: 197-203, 2006.
<br><br>
Mujynya-Ludunge K, Viswambharan H, Driscoll R, Ming XF, von Segesser LK, Kappenberger L, Yang Z, Vassall G: Endothelial nitric oxide synthase gene transfer restores endothelium-dependent relaxations and attenuates lesion formation in carotid arteries in apolipoprotein E-deficient mice. Basic Res. Cardiol. 100: 102-111, 2005.
<br><br>
Nachtigal P, Kopecky M, Solichova D, Zdansky P, Semecky V: The changes in the endothelial expression of cell adhesion molecules and iNOS in the vessel wall after the short-term administration of simvastatin in rabbit model of atherosclerosis. J. Pharm. Pharmacol. 57: 197-203, 2005.
<br><br>
Nematbakhsh M, Hayat-Davoodi P, Rajabi P, Samarian SH: The effect of estrogen on endothelial permeability of aorta and the level of serum nitrite concentration in cholesterol-fed ovariectomized rabbit. IranBiomed. J. 6: 77-82, 2002.
<br><br>
Nicotera P, Bonfoco E, Brune B, Tarr JM, Eggleton P, Winyard PG: Mechanisms for nitric oxide-induced cell death: involvement of apoptosis. Adv. Neuroimmunol. 5: 411-20, 1995.
<br><br>
Preli RB, Klein KP, Herrington DM: Vascular effects of dietary L-arginine supplementation Atherosclerosis. 162: 1-15, 2002.
<br><br>
Siasos G, Tousoulis D, Antoniades C, Stefanadi E, Stefanadis C: L-arginine the substrate for NO synthesis: an alternative treatment for premature atherosclerosis? Int. J. Cardiol. 116: 300-8, 2007.
<br><br>
Stefanec T: Endothelial apoptosis: could it have a role in the pathogenesis and treatment of disease? Chest 117: 841-54, 2000.
<br><br>
Suzuki T, Hayase M, Hibi K, Hosokawa H, Yokoya K, Fitzgerald PJ, Yock PG, Cooke JP, Suzuki T, Yeung AC: Effect of local delivery of L-arginine on in-stent restenosis in humans. Am. J. Cardiol. 89: 363-367, 2002.
<br><br>
Taylor BS, Geller DA: Molecular regulation of the human inducible nitric oxide synthase (iNOS) gene. Shock 13: 413-24 , 2000.
<br><br>
Tousoulis D, Boger RH, Antoniades C, Siasos G, Stefanadi E, Stefanadis C: Mechanisms of disease: L-arginine in coronary atherosclerosis--a clinical perspective. Nat. Clin. Pract. Cardiovasc. Med. 4: 274-83, 2007.
<br><br>
Wang BY: Dietary arginine prevents atherogenesis in the coronary artery of hypercholesterolemic rabbits. J. Am. Coll. Cardiol. 23: 452-458, 1994.
<br><br>
Wang X, Cade R, Sun Z: Expression of human eNOS in cardiac and endothelial cells. Methods Mol. Med. 112: 91-107, 2005.
<br><br>
Wilcox JN, Subramanian RR, Sundell CL, Tracey WR, Pollock JS, Harrison DG, Marsden PA: Expression of multiple isoforms of nitric oxide synthase in normal and atherosclerotic vessels. Arterioscler. Thromb. Vasc. Biol. 17: 2479-2488, 1997.<br>
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