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		<PublisherName>University of South Bohemia, Ceske Budejovice and Versita, Warsaw</PublisherName>
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			<JournalPrintISSN>1214-021X</JournalPrintISSN>
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			<JournalTitle>Journal of Applied Biomedicine</JournalTitle>
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				<VolumeNumber>8</VolumeNumber>
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						<CoverDate Year="2010" Month="6" Day="1"/>
						<CoverDisplay>Number 2 / June 2010</CoverDisplay>
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					<IssueID>LR323T458627</IssueID>
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				<Article ArticleType="Original">
					<ArticleInfo Free="No" ESM="No">
						<ArticleDOI>10.2478/v10136-009-0011-y</ArticleDOI>
						<ArticlePII>12P0W30362616667</ArticlePII>
						<ArticleSequenceNumber>4</ArticleSequenceNumber>
						<ArticleTitle Language="En">Circadian variations in biochemical markers of bone metabolism in horse of different age</ArticleTitle>
						<ArticleFirstPage>73</ArticleFirstPage>
						<ArticleLastPage>79</ArticleLastPage>
						<ArticleHistory>
							<RegistrationDate>20100524</RegistrationDate>
							<ReceivedDate>20100524</ReceivedDate>
							<Accepted>20100524</Accepted>
							<OnlineDate>20100524</OnlineDate>
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						<FullTextFileName>12P0W30362616667.pdf</FullTextFileName>
						<FullTextURL>http://versita.metapress.com/link.asp?target=contribution&amp;id=12P0W30362616667</FullTextURL>
						<Composite>2</Composite>
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					<ArticleHeader>
						<AuthorGroup>
							<Author AffiliationID="A1">
								<GivenName>Claudia</GivenName>
								<Initials/>
								<FamilyName>Giannetto</FamilyName>
								<Degrees/>
								<Roles/>
							</Author>
							<Author AffiliationID="A1">
								<GivenName>Stefania</GivenName>
								<Initials/>
								<FamilyName>Casella</FamilyName>
								<Degrees/>
								<Roles/>
							</Author>
							<Author AffiliationID="A1">
								<GivenName>Francesco</GivenName>
								<Initials/>
								<FamilyName>Fazio</FamilyName>
								<Degrees/>
								<Roles/>
							</Author>
							<Author AffiliationID="A1">
								<GivenName>Vanessa</GivenName>
								<Initials/>
								<FamilyName>Messina</FamilyName>
								<Degrees/>
								<Roles/>
							</Author>
							<Author AffiliationID="A1">
								<GivenName>Giuseppe</GivenName>
								<Initials/>
								<FamilyName>Piccione</FamilyName>
								<Degrees/>
								<Roles/>
							</Author>
							<Affiliation AFFID="A1">
								<OrgDivision/>
								<OrgName>Department of Experimental Sciences and Applied Biotechnology, Laboratory of Veterinary Chronophysiology, Faculty of Veterinary Medicine, University of Messina, Italy</OrgName>
								<OrgAddress/>
							</Affiliation>
						</AuthorGroup>
						<Abstract Language="En">The author studied in thirty Thoroughbred horses the influence of age and gender on daily rhythms of serum osteocalcin (OC). The animals were divided into two groups. Group A: six male and six female 2 years old; Group B: six male, six female and six geldings 6 years old. They were housed individually in box-like stalls under natural photoperiod and environmental conditions. Blood samples were collected every 3 hours over a 48 hour period. Statistical differences of serum OC concentration due to different gender were observed in both Groups A and B. Daily rhythms of serum OC concentration were observed only in Group B, with nocturnal acrophase. In females the acrophase was statistically postponed for 1 hour compared to male and gelding. Male and female showed a more robust daily rhythm than the geldings. The results showed that blood sampling for determination of serum OC should be strictly standardized with regard to the time of the day.</Abstract>
						<KeywordGroup Language="En">
							<Keyword>circadian rhythms</Keyword>
						</KeywordGroup>
						<KeywordGroup Language="En">
							<Keyword>osteocalcin</Keyword>
						</KeywordGroup>
						<KeywordGroup Language="En">
							<Keyword>age</Keyword>
						</KeywordGroup>
						<KeywordGroup Language="En">
							<Keyword>gender</Keyword>
						</KeywordGroup>
						<KeywordGroup Language="En">
							<Keyword>horses</Keyword>
						</KeywordGroup>
						<biblist>
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						</biblist>
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