SUMMARY
Bone marrow mensenchyme cells(BMSCs) can differentiate into endothelial progenitor cells which then migrate to injured sites for the repair of
neointima, and stromal cell-derived factor-1alpha (SDF-1alpha) can mediate the migration of CXCR4 expressing stem/progenitor cells to injured
sites for repair. Protein and mRNA expression of SDF-1alpha and CXCR4 were determined by RT-PCR, Western blot and ELISA. Immediately after common
carotid artery balloon injury, the mRNA expression of SDF-1alpha in vascular smooth muscle cells(VSMCs) first increased and then decreased 7 days
later. VSMCs transfected with SDF-1alpha siRNA did not express SDF-1alpha mRNA, but after transfection with SDF-1alpha siRNA, the SDF-1alpha
content in injured VSMCs gradually returned to the baseline level. Normal BMSCs rarely expressed CXCR4 mRNA, but the CXCR4 mRNA expression on
BMSCs increased significantly 4 days after common carotid artery injury and was maintained. The migration of BMSCs after artery injury was
enhanced when compared with normal BMSCs, but SDF-1alpha siRNA transfection of VSMCs and AMD3100 treatment remarkably decreased the chemotaxis of
BMSCs to VSMCs and SDF-1alpha, respectively. We conclude that the SDF-1alpha/CXCR4 axis plays an important role in the migration of BMSCs after
balloon injury and can ultimately cause abnormal proliferation of the intima.
KEY WORDS
bone marrow mensenchyme cells; vascular smooth muscle cells; stromal cell-derived factor-1alpha
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